Can you sterile filter fbs

Advanced Search. Fetal Bovine Serum. Fetal Bovine Serum FBS —also called fetal calf serum FCS —is the growth supplement of choice when performing routine and technically-demanding cell culture techniques. Most cell types and cell culture systems will benefit from the inclusion of FBS in growth media. Advantages of using fetal bovine serum compared to adult bovine serum include both the lower level of endogenous immunoglobulin present in the serum and increased levels of growth factors.

Not only does this result in superior growth profiles for eukaryotic cells, it is an advantage in reducing antibody cross reactivity between the cell culture supplement and the cells growing in culture.

The use of FBS is a significant benefit when growing, screening or subcloning hybridoma cells engineered to secrete specific monoclonal antibodies. In order to offer you the best possible experience, Rockland uses cookies. Do not use this serum. We recommend thawing the serum at oC. However, if necessary, you may thaw the serum at room temperature. Swirl the bottles gently to mix the serum during the thawing process. The color of FBS is brown to brown-red. It is dependent mainly on the hemoglobin concentration in the specific lot.

The color does not affect the FBS performance. Deformation does not affect the FBS quality and performance. Cookie Policy This website uses cookies to ensure proper functionality of the shopping cart and checkout progress.

Accept Decline. Contact us. Biological Industries. Our History. Search: Enter keyword or catalog number. Fetal Bovine Serum. However, if you happen to be the type of person like one of my colleagues, you can thaw another tube and add the extra 5mL. A bit overboard if you ask me, but to each their own. Thaw all the FBS in the hood, re-aliquot into 45mL and put a big note to remind everyone that they must filter the media when they add this FBS. No, i think you misunderstand me.

I don't know why, and how to deel with it. Thanks for so much concern. But may you give some advise about settling the problem? To get rid of the precipitate in FBS, you may wish to use a filter paper.

Then you can filter it with a 0. Maybe you are right in a way. However i don't agree to do that, i don't know how much nutrient will lose after doing that.

And if it can be used to culture cells still leave to be tested. Forum Index Home Live Discussion. Pages: 1 2 Next. Go to the forum page Printer Friendly Version.